|
KMID : 0371320070730010008
|
|
Journal of the Korean Surgical Society
2007 Volume.73 No. 1 p.8 ~ p.12
|
|
|
Comparison of Intra-gall Bladder and Intra-common Bile Duct Injection of Collagenase Solution for Mouse Pancreatic Islet Isolation
|
|
Park Jong-Kwon
|
|
|
Abstract
|
|
|
Purpose: A high yield of viable pancreatic islets is an essential prerequisite for the study of pancreatic islet transplantation. The purpose of this study is to compare the yield between intra-gall bladder (intra-GB) and intra-common bile duct (intra-CBD) injection of collagenase solution for isolation of mouse pancreatic islets.
Methods: The mice were divided into two groups, the intra-GB and intra-CBD groups, and each group included
twelve mice, respectively. Collagenase solution was injected via the gallbladder in the intra-GB group mice, while this was done via the common bile duct in the intra-CBD group. After removal and digestion of the mouse pancreases, the pancreatic islets were isolated by Ficoll density gradient centrifugation and hand picking.
Results: The intra-GB group yielded 121.67±39.86 IEQs, and the intra-CBD group reveled 168.17±29.23 IEQs. There was a statistically significant difference in islet yield between the two groups (P=0.005, Mann-Whitney Test). The purities of the isolated islets were 86.42±3.99% for the intra-GB group and 87.17±4.47% for the intra-CBD group, and there
was no difference between the two groups (P=0.755, Mann- Whitney Test).
Conclusion: Both the intra-GB and intra-CBD groups yielded an average of ＞120 IEQs. However, the intra-CBD group revealed a higher yield than the intra-GB group for isolating mouse pancreatic islets.
|
|
|
KEYWORD
|
|
|
Pancreatic islet, Gall bladder, Common bile duct
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
  
|
|